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Application of high-speed counter-current chromatography for the isolation of sulforaphane from broccoli seed meal

Report of a novel method of using high-speed countercurrent chromatography to produce pure sulforaphane from broccoli seed meal.

Year of Publication2008

In order to produce large amounts of pure sulforaphane for research purposes, a novel method using high-speed countercurrent chromatography (HSCCC) was developed. Without any initial cleanup steps, sulforaphane was successfully purified from the ethyl acetate extract of the broccoli seed meal by HSCCC. The separation was performed with two-phase solvent systems: n-hexane/ethyl acetate/methanol/water (1:5:1:5, v/v/v/v). From 850 mg of the ethyl acetate extract, 186 mg of sulforaphane was isolated with the solvent system. The purified compound was over 97% purity as determined by HPLC analysis, and the chemical structure was confirmed by MS and 1H and 13C NMR.

Citation

Liang, H., Li, C., Yuan, Q. and Vriesekoop, F. (2008) "Application of high-speed counter-current chromatography for the isolation of sulforaphane from broccoli seed meal"Journal of Agricultural and Food Chemistry 56 (17) pp 7746-7749

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Supporting the development of the national rural economy.

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