An investigation of wound healing in sugar beet roots using light and fluorescent microscopy

The declared crop area for sugar beet in the UK in 2000 was 169,530 ha making it the UK's second most important crop in terms of production. The aim of this study was to characterize the wound healing events in developing and mature sugar beet and also to compare the effects of commercial harvesting and subsequent storage on bruising in sugar beet.

Year of Publication2001

Following impact, wound healing was investigated in roots of sugar beet using fluorescence microscopy in conjunction with a conventional lignin test. Samples of sugar beet roots were harvested at different stages of development and impacted in the laboratory with a falling bolt delivering 1–4 Joules. A response in the form of deposition of brown material, presumed to be melanin, along the outer and inner walls of cells at the wound surface was observed within 3 d of impact. This material eventually became granular in appearance. Formation of a ligno-suberized boundary layer from cells present at the time of impact first occurred in 16-week-old roots 9 d after impact. Intensity o calcofluor fluorescence supported the findings made using light microscopy. As wound healing progressed with time, the intensity of calcofluor fluorescence declined, demonstrating interference by wound healing products with calcofluor binding. Aggressive harvest and subsequent storage dramatically reduced calcofluor fluorescence indicating that this dye may have potential value in the assessment of tissue damage.


Ibrahim, L ; Spackman, VMT ; Cobb, AH (2001) "An investigation of wound healing in sugar beet roots using light and fluorescent microscopy"Annals of Botany 88 (2) pp 313-320

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Additional keywords/tags

fluorescenceimpact damage bruisingligninsugar beetwound healing
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