OpenFields

Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida

This paper describes a cDNA clone which encodes an amino-acid protein similar to annexins in other nematode species. The possible role that this protein might play in the infection process is discussed.

Year of Publication2001

A full length cDNA clone was isolated by screening a mixed stage Globodera pallida expression library using the monospecific polyclonal antibody IACR-PC320. Sequence comparison indicated that the predicted 332 amino acid protein encoded by the cDNA was a member of the annexin gene family named gp-nex and very similar to annexins found in other nematode species. Annexins are calcium-dependent phospholipid binding proteins characterised by four repeated domains approximately 70 amino acids in length. The predicted amino acid sequence of the protein did not contain an N-terminal secretion signal peptide; however, the protein was shown to be present in excretory/secretory products from G. pallida second stage juveniles treated with the neurotransmitter 5 methoxy-N, N dimethyl tryptamine. Gp-nex encodes a protein of 35 kDa and was immunolocalised in the amphids, genital primordium and in the constraining muscles above and below the metacorpus pump chamber of G. pallida second stage juveniles. It is also present in eggs and adult females of the two species of the potato cyst nematodes.

Citation

Fioretti, L; Warry, A; Porter, A; Haydock, P and Curtis, R (2001) "Isolation and localisation of an annexin gene (gp-nex) from the potato cyst nematode, Globodera pallida"Nematology 3 (1) pp 45-54

This item is categorised as follows

Additional keywords/tags

potato cyst nematodesecretiongenital primordiumamphidscaenorhabditis elegans
Organisation Logo for Harper Adams University

Supporting the development of the national rural economy.

Website

What Next...?

This is a brief summary of an item in the OpenFields Library. This free online library contains items of interest to practitioners and researchers in the agricultural and landbased industries.